monocyte-like cell line
نویسنده
چکیده
The complement fragment C5a and its des-Arg74 derivative, C5a des Arg, are potent mediators of leucocyte chemotaxis and are thought to play an important role in the accumulation of leucocytes at sites of inflammation. Although specific receptors for C5a have been demonstrated on murine macrophages (Chenoweth et al., 1982) and human monocytes (Chenoweth & Goodman, 1983; Marder et al., 1985), most work to date has concentrated on the human neutrophil CSa receptor due to the relative abundance and ease of purification of this cell type. Using a monocyte-like cell line, U-937, as a model of the monocyte response to C5a des Arg, we show here the level ofCSa des Arg receptor expression appears to be regulated by the extracellular concentration of C5a des Arg itself. This may provide a mechanism by which the cell is able to maintain a chemotactic response over a wide range of attractant concentrations.
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